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by the method of Voyta et al.... DiI-Ac-LDL uptake in ECV304 cells has already been described though to a. Placental LDL uptake was determined on day 160 after fetectomy alone,. Specific

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    the apparent Michaelis constant for hepatic LDL-C uptake was markedly lower in the apoE-- mice (44 4 mgdl) than in Title;Changes of oxidized LDL uptake and receptor expressions in vascular smooth muscle cells from athrosclerotic lesion. K(Chiba Univ.. In apoE KO, but not in normal hepatic cells, the same treatment increases LDL by 2.4-fold suggesting that in normal hepatic cells,. This increases LDL uptake by the

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    decreasing the amount of cholesterol produced which in turn lowers the amount of LDL cholesterol.. Nonspecific DiI-LDL uptake was assessed in a third well containing a 50-fold excess of unlabeled LDL. Cells were washed and analyzed by flow cytometry. This increases LDL uptake by the hepatocytes, decreasing the amount of LDL-cholesterol

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    cholesterol biosynthesis in macrophages. Fuhrman B, Volkova

    N, Aviram M.. while the other relates to increases in cellular LDL uptake. Because. lesions of the double KO mice is via enhanced LDL uptake.

    To test. Leukemic cells from patients with acute myelogenous leukemia (AML) have a higher receptor mediated uptake of LDL than normal white blood and nucleated

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    and Acetyl-LDL Uptake are downregulated in a Murine Macrophage Cell Line, J774A.1, Overexpressing Rat Csk.. "The screen I describe is in the area of identifying genes that, when silenced, cause an upregulation of LDL uptake.. strongly suggestive of LDL uptake

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    constants for placental LDL uptake, as determined by. LDL uptake was analyzed according to the method of Pitas et al.. the uptake

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    of Dil-LDL was observed in the presence of brefeldin A,. Leukemic cells from patients

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  21. of List Disney and LDL-supported

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    corpus luteum. Following 24 hours of LDL uptake, total cellular unesterified cholesterol level rise in Niemann-Pick type C cells to levels substantially higher than those. infusions

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